• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Method for the precise dilution of a liquid sample, in which diluting agent is pumped through a main channel (1) open at its lower tip and, at the same time, sample is pumped through a side channel (2), whose lower tip (11) opens into the main channel. The invention can be used e.g. in photometers.
    公开号:SU1584761A3
    申请号:SU843814610
    申请日:1984-11-26
    公开日:1990-08-07
    发明作者:Экхольм Пертти；Кауканен Эско
    申请人:Лабсистемз Ой (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to a method for transferring or accurately diluting liquids and can be used in the construction of photometers and fluorometers.
    The purpose of the invention is to improve the accuracy and efficiency of sampling.
    The method consists in that the side channel present in the sampler fills the sample, the main channel is filled with a diluent by connecting the pressure source, the sample is forced out of the side channel to the main channel, and the diluent is displaced from the main channel bottom tip.
    Figure 1 shows a schematic diagram of a device implementing the proposed method; figure 2 - coaxial arrangement of the tubes of the main and side channels in the tip of the liquid dispenser.
    A series of diluted i liquid samples are prepared. The main channel 1 is filled with water, and the side channel 2 is filled with the test liquid. Depending on the required degree of dilution of the sample of the test liquid, the dispenser channels may have a different structure. Dispenser channels can be filled with various fluids. For example, instead of water, the main channel can be filled with the required reagent.
    The operation of the device begins with the filling of both channels 1 and 2 and the connecting channel 3 with water, which can be done by injecting
    SP
    00 v vj
    Uh
     U4
    3158
    water tubular pump 4 from the container 5 or the water tank 6 with simultaneous or sequential opening of the valves 7 and 8, when the piston 9 is in its lowest position. Then, from the reservoir 10, the test liquid is sucked into the side channel 2, for which the valves 7 and 8 are closed and the piston 9 is moved backwards. The amount of test liquid sucked into the side channel exceeds the amount needed to prepare several doses, since the upper part of the column of test liquid (at the end of the side channel) is diluted with water in the common part of the channels and water that is retained on the walls of the side channel . After the piston 9 stops moving, the valve 7 opens and water is displaced 6 in such quantity that the common part of the channels is free from the liquid under study. Thus, the column of the test liquid is accurately cut off at the end 11 of the side channel 2.
    The dilution of the test liquid in the reservoir 12 is performed as follows: the valve 7 remains open and the valve 8 is closed. The tubular pump 4 supplies water to the reservoir 12. At the same time or later, the test liquid is displaced from the side channel into the common part of both channels by the movement of the piston 9, from where it enters the mixing tank 12 with water, and at that time the liquid under investigation mixes with water. The required degree of dilution of the test liquid is achieved by an appropriate choice of the ratio of pumping rates and / or ratio of pumping cycles. Next, the piston 9 stops, and after the common part of the channels is freed from the liquid, the tubular pump 4 also stops. After that, the dispenser tip 13 can be installed above the new mixing tank. Similarly, a new mixing tank can be installed under the dispenser tip. The following diluted sample of the test fluid is prepared similarly, with the degree of dilution of the test fluid dose-to-dose varying.
    o
    0
    five
    The proposed method provides high accuracy in the dosing of the test liquid, since the cut-off of the column of the test liquid occurs at the end of the side channel, surrounded on all sides by the diluting liquid. Thus, the proposed dosing method eliminates undesirable phenomena at the end of the side channel, such as a bulge or concavity of the surface of the test liquid at the channel end, splashing the test liquid on the outer surface of the channel end, which can lead to a corresponding decrease in the dose of the test liquid or the corresponding increase the next dose of fluid. If the end of the channel is surrounded by air, the liquid under investigation may fall mechanically by wiping or washing the end of the channel with liquid, but in this case it is not possible to predict the loss of the test liquid from the tube forming the side channel. In addition, when washing the outer surface of the end of the side channel, a washing liquid may remain on the surface of the latter, diluting the subsequent dose of the test liquid at the site of its collection.
    Since the water and the test liquid at the end of the tube, which is filled with the test liquid, are in direct contact, their mutual diffusion occurs, but the effects of such diffusion can be minimized if the dosing cycle is accelerated and the cycle duration is kept constant. dosage. Mixing the initial dose of the test liquid, which is sucked 45 into the receiving tube first, with water can be prevented by the formation of an air bubble between the water and the test liquid or by introducing a small volume of auxiliary liquid between the water and the test liquid, which does not mix and react neither with water nor with the test liquid.
    An additional advantage of the method of dosing a liquid is that it provides the het with good mixing of the test liquid with water, which occurs in the common part of the channels and during the displacement of the mixture
    thirty
    40
    50
    55
    515
    water and the test liquid in the mixing tank. In many cases of mixing, it is quite sufficient and a special operation of mixing water and liquid under study is not required.
    The proposed method allows the use of concentrated reagents, since if such a reagent is introduced into the channel for the test liquid, it is diluted at the outlet of the dispenser to the desired concentration. Such a diluted to the desired concentration of reagent is introduced into various cuvettes, and the degree of dilution of the reagent from cycle to cycle may vary. The dosage method can also be used to dose 1 mixture - two reagents that can only be mixed immediately before use. In this case, one reagent is introduced into the main channel of the dispenser, and the other reagent is introduced into the side channel.

    3. Method nonn.i and 2, characterized in that the diluent is injected from above through the main channel equipped with a valve.
    4. A method according to claims 1 to 3, characterized in that, simultaneously with the filling of the main channel with the diluting liquid, the lower part of the side channel is filled with the diluting liquid, after which a sample of the test liquid is fed into the side channel,
    5. A method according to Claims 1-4, characterized in that after filling the lateral cocoa with the diluting liquid, a bubble of neutral gas or a drop of neutral liquid is supplied to this channel.
    6. A device for diluting liquid samples containing a sampler with a main channel with an open lower tip and a side channel, o t
    权利要求:
    Claims (2)
    [1]
    Invention Formula
    1. A method for diluting liquid samples in a sampler with a main and side channels, including filling the side channel with a sample, forcing the diluent substance into the main coaxially located channel by connecting a pressure source and displacing the sample from the side channel to the main channel, increase the accuracy and efficiency of sampling, to displace the diluent from the main channel
    and the samples into the main channel are filled with the diluent in the main channel, and the diluent is displaced from the main channel through the lower tip.
    2. The method according to claim 1, is characterized by the fact that the diluent is injected into the main channel by suction through the lower tip.
    [2]
    in order to improve the accuracy and efficiency of sampling, the side channel is made stepped and its lower opening is placed B at the tip of the main channel coaxially,
    7. The device according to claim 6, that is, that it is provided with a connecting channel with a valve located between the side and equipped with a valve main channel ..
    8. The device according to PP. 6 and 7,
    It is characterized in that the side channel is provided with a piston located at the junction of the side and connecting channels.
    9. The device according to PP.6 - 8, about T - is characterized by the fact that the lower
    the tip of the main channel is made in the shape of a truncated cone.
    10. The device according to PP.6 - 9, about T - is characterized by the fact that the bottom
    the end of the side channel is cylindrical.
    Editor I.Gorn
    Compiled by L. Nechiporenko Tehred M. Didyk
    Order 2267
    Circulation 495
    VODIL of the State Committee for Inventions and Discoveries at the State Committee on Science and Technology of the USSR 4/5, Moscow, Zh-35, Raushsk nab. 113035
    I
    13
    FIG. 2
    Proofreader M.Kucher va
    Subscription
    类似技术:
    公开号 | 公开日 | 专利标题
    SU1584761A3|1990-08-07|Method and apparatus for diluting liquid samples
    US4888998A|1989-12-26|Sample handling system
    US6066298A|2000-05-23|Pretreatment apparatus
    JP3256818B2|2002-02-18|Sample liquid analyzer and sample liquid analysis method
    US5474744A|1995-12-12|Automatic pipetting device with cleaning mechanism
    US5079959A|1992-01-14|Analyzing system using sheath flow of sample
    US4242909A|1981-01-06|Sample injector
    US6706245B2|2004-03-16|Three stage needle for use with an autosampler
    AU629767B2|1992-10-08|Combined closed and open tube sampling apparatus and method
    US20050279387A1|2005-12-22|Probe washing cups and methods
    EP0022654A1|1981-01-21|Liquid handling device
    US3901653A|1975-08-26|Liquid sampling device
    DE2211032A1|1973-09-20|METHOD AND DEVICE FOR DETERMINING GASES SOLVED IN A LIQUID, IN PARTICULAR IN THE BLOOD
    EP0253519B1|1991-10-09|Sample handling system
    US4002269A|1977-01-11|Liquid proportioning system in a liquid sample analyzer
    US4245509A|1981-01-20|Sampling apparatus
    GB1560063A|1980-01-30|Apparatus for repeated liquid sampling
    US3649218A|1972-03-14|Method for preparing metered liquid sample dilutions
    US5849592A|1998-12-15|Carrierless sequential injection analysis
    IL31283A|1972-02-29|Liquid mixing and transfer structure and method
    US3747412A|1973-07-24|Sample mixing and metering apparatus
    RU2730922C2|2020-08-26|Device and method for high-accuracy sampling of liquids in an automatic sample analyzer
    CN109110856A|2019-01-01|Oils auto extractive system and analysis system in a kind of water
    US4014652A|1977-03-29|Automatic analytic apparatus of liquids
    RU2616657C1|2017-04-18|Apparatus for collecting and diluting portions of radioactive solution
    同族专利:
    公开号 | 公开日
    AT50061T|1990-02-15|
    DE3481219D1|1990-03-08|
    JPS60135766A|1985-07-19|
    FI834387A0|1983-11-30|
    EP0144134A3|1987-07-15|
    US4610170A|1986-09-09|
    EP0144134B1|1990-01-31|
    JPH0324988B2|1991-04-04|
    EP0144134A2|1985-06-12|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    RU2485473C2|2011-03-31|2013-06-20|Феликс Эргардович Гофман|Apparatus for collecting and diluting portions of radioactive solution |GB741788A|1953-12-07|1955-12-14|British Transp Commission|Improvements in or relating to devices for taking samples of liquids|
    US2980512A|1958-03-28|1961-04-18|Bayer Ag|Continuously working plant colorimeter|
    US3197285A|1961-05-08|1965-07-27|Rosen Sidney|Sampling machine|
    GB1249103A|1967-07-19|1971-10-06|Atomic Energy Authority Uk|Improvements in or relating to analytical apparatus|
    FR1585020A|1968-05-08|1970-01-09|
    US3764041A|1970-10-26|1973-10-09|Searle & Co|Microdispensing process and apparatus|
    US3668936A|1970-12-15|1972-06-13|Technicon Instr|Method and apparatus for sampling|
    US3831618A|1972-12-22|1974-08-27|Abbott Lab|Apparatus for the precision metering of fluids|
    US3869068A|1974-06-06|1975-03-04|Hyperion Inc|Diluter probe assembly|
    US3960020A|1974-12-30|1976-06-01|Technicon Instruments Corporation|Liquid aspirating probe assembly of a supply analyzer|
    US3955930A|1975-04-07|1976-05-11|Justin Joel Shapiro|Automatic dilutor having coupled diluent and reagent plungers|
    DE2602675C3|1976-01-24|1981-05-21|Bodenseewerk Perkin-Elmer & Co GmbH, 7770 Überlingen|Method and device for the automatic implementation of series analyzes|
    US4141469A|1977-05-02|1979-02-27|Lee Thomas E|Microdispensing dilution system|
    US4228831A|1978-12-11|1980-10-21|Abbott Laboratories|Probe and syringe drive apparatus|
    US4318885A|1979-09-10|1982-03-09|Olympus Optical Co., Ltd.|Liquid treating device for chemical analysis apparatus|
    US4327595A|1980-07-07|1982-05-04|Hamilton Company|Method and apparatus for simultaneous dilution and dispensation|
    US4476734A|1983-03-07|1984-10-16|International Business Machines Corporation|Wet needle sampler for use with a gas chromatograph|
    FR2553891A1|1983-10-25|1985-04-26|Applic Tech Materiaux|Device for diluting a product, particularly during titration|US4871682A|1986-04-30|1989-10-03|Baxter Travenol Laboratories, Inc.|Diluent carryover control|
    US4868129A|1987-08-27|1989-09-19|Biotrack Inc.|Apparatus and method for dilution and mixing of liquid samples|
    US4946795A|1987-08-27|1990-08-07|Biotrack, Inc.|Apparatus and method for dilution and mixing of liquid samples|
    DE3886140T2|1987-08-27|1994-05-19|Biotrack Inc|Device and method for diluting and mixing liquid samples.|
    US5077017A|1987-11-05|1991-12-31|Biotrack, Inc.|Integrated serial dilution and mixing cartridge|
    DE4017868A1|1990-06-02|1990-10-31|Siegfried Dipl Phys Stiller|Mixing chamber - for dilution and haemolysis of blood free from air bubbles|
    US5073505A|1990-06-27|1991-12-17|United Technologies Corporation|Eluant storage and preparation apparatus and method for using the same in a zero gravity environment|
    DE4130742A1|1991-09-16|1993-03-18|Inst Diabetestechnologie Gemei|METHOD AND ARRANGEMENT FOR DETERMINING THE CONCENTRATION OF INGREDIENTS IN BODY LIQUIDS|
    US5741709A|1994-03-30|1998-04-21|Industrial Technology Research Institute|Multiple injection analysis|
    WO2000051737A1|1999-03-05|2000-09-08|Hitachi Chemical Co., Ltd.|Self-washable nozzle tip|
    JP4174599B2|2003-07-08|2008-11-05|株式会社島津製作所|High-performance liquid chromatograph fractionator|
    WO2006012454A1|2004-07-22|2006-02-02|Amgen Inc.|Method and apparatus for forming a dilution by fluid dispersion|
    EP2660482B1|2005-08-22|2019-08-07|Life Technologies Corporation|Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen|
    US9658143B1|2013-03-15|2017-05-23|Elemental Scientific, Inc.|Offline mixing|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    FI834387A|FI834387A0|1983-11-30|1983-11-30|FOERFARANDE FOER UTSPAEDNING AV VAETSKEPROV|
    [返回顶部]